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Addgene

pDA025
(Plasmid #131384)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131384 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Modifications to backbone
    Hygromycin replaced with Puromycin, CMV replaced with Tet (2nd) promoter
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ORFeus
  • Species
    H. sapiens (human)
  • Mutation
    H230A En
  • Tag / Fusion Protein
    • ORF2-3xFlag

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDA025 was a gift from Kathleen Burns (Addgene plasmid # 131384 ; http://n2t.net/addgene:131384 ; RRID:Addgene_131384)

  • For your References section:

    Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, Smogorzewska A, Holland AJ, Burns KH. Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 10.1038/s41594-020-0372-1. Epub 2020 Feb 10. 10.1038/s41594-020-0372-1 PubMed 32042151
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