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Addgene

pDC21
(Plasmid #131367)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131367 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    meg-3 (aa1-544)
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1595
  • Entrez Gene
    meg-3 (a.k.a. CELE_F52D2.4)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDC21 was a gift from Geraldine Seydoux (Addgene plasmid # 131367 ; http://n2t.net/addgene:131367 ; RRID:Addgene_131367)

  • For your References section:

    Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3. Smith J, Calidas D, Schmidt H, Lu T, Rasoloson D, Seydoux G. Elife. 2016 Dec 3;5. pii: 21337. doi: 10.7554/eLife.21337. 10.7554/eLife.21337 PubMed 27914198
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