HoxC-downstream-5xTetO Donor
(Plasmid
#131341)
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PurposeDonor template to insert 5xTet operator sequence into downstream of HoxC cluster.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR™-Blunt vector
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Backbone manufacturerThermoFisher
- Backbone size w/o insert (bp) 3500
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Vector typeMouse Targeting, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHoxC-downstream-5xTetO Donor
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SpeciesM. musculus (mouse)
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Insert Size (bp)20
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HoxC-downstream-5xTetO Donor was a gift from Danny Reinberg (Addgene plasmid # 131341 ; http://n2t.net/addgene:131341 ; RRID:Addgene_131341) -
For your References section:
Capturing the Onset of PRC2-Mediated Repressive Domain Formation. Oksuz O, Narendra V, Lee CH, Descostes N, LeRoy G, Raviram R, Blumenberg L, Karch K, Rocha PP, Garcia BA, Skok JA, Reinberg D. Mol Cell. 2018 Jun 21;70(6):1149-1162.e5. doi: 10.1016/j.molcel.2018.05.023. 10.1016/j.molcel.2018.05.023 PubMed 29932905