pcDNA3.1-N-Myc_535-888 USP7
(Plasmid #131248)

• Purpose: Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.
• Depositing Lab: Roger Woodgate
• Publication: Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. doi: 10.1016/j.jmb.2020.166733. Epub 2020 Dec 3.

Backbone

• Vector backbone: pcDNA3.1+/N-Myc
• Backbone manufacturer: Genscript
• Backbone size w/o insert (bp): 5435
• Total vector size (bp): 7154
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin carboxyl-terminal hydrolase 7
• Alt name: Ubiquitin-specific-processing protease 7
• Species: H. sapiens (human)
• Insert Size (bp): 1719
• Mutation: A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7 plasmid (Addgene plasmid 131246), where the codon for E889 was converted to a STOP codon. This mutation limits expression to UBLs 1 - 3
• Entrez Gene: USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pCDNA3.1-N-Myc_535-888 USP7.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry