pSaCas9-sgRNA-Tetra-com vector
(Plasmid
#131227)
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PurposeFor expressing gRNA, whose Tetraloop is replaced with a com aptamer
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepX601
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namevector for com modified sgRNA
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gRNA/shRNA sequencevector
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSaCas9-sgRNA-Tetra-com vector was a gift from Baisong Lu (Addgene plasmid # 131227 ; http://n2t.net/addgene:131227 ; RRID:Addgene_131227) -
For your References section:
Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing. Lyu P, Javidi-Parsijani P, Atala A, Lu B. Nucleic Acids Res. 2019 Jul 12. pii: 5531787. doi: 10.1093/nar/gkz605. 10.1093/nar/gkz605 PubMed 31299082