pcDNA5-FRT-TO-NOD2-EGFP
(Plasmid #131207)

• Purpose: mammalian expression, tet R
• Depositing Lab: Thomas Kufer
• Publication: Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi: 10.15252/embj.2020106272. Epub 2021 May 4.

Backbone

• Vector backbone: pcDNA5-FRT-TO
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NOD2
• Species: H. sapiens (human)
• Insert Size (bp): 3134
• Entrez Gene: NOD2 (a.k.a. ACUG, BLAU, BLAUS, CARD15, CD, CLR16.3, IBD1, NLRC2, NOD2B, PSORAS1, YAOS)
• Promoter: pCMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For cloning Nod2, variant 1 was used. Nod1wt was cut out and Nod2 without stop codon was integrated. To ensure that EGFP and Nod2 are in-frame, two additional bases were added at reverse primer (primer without stop codon of Nod2).

Insert has three silent mutations:
Codon 178: TCG instead of TCC --> Serine
Codon 587: CCT instead of CCC --> Proline
Codon 792: CGG instead of CGT --> Arginine