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pcDNA5-FRT-TO-EGFP-RIP2_Y474F
(Plasmid #131204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5-FRT-TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RIPK2 Y474F
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Mutation
    Y474F
  • Entrez Gene
    RIPK2 (a.k.a. CARD3, CARDIAK, CCK, GIG30, RICK, RIP2)
  • Promoter pCMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT-TO-EGFP-RIP2_Y474F was a gift from Thomas Kufer (Addgene plasmid # 131204 ; http://n2t.net/addgene:131204 ; RRID:Addgene_131204)

  • For your References section:

    XIAP controls RIPK2 signaling by preventing its deposition in speck-like structures. Ellwanger K, Briese S, Arnold C, Kienes I, Heim V, Nachbur U, Kufer TA. Life Sci Alliance. 2019 Jul 26;2(4). pii: 2/4/e201900346. doi: 10.26508/lsa.201900346. Print 2019 Aug. 10.26508/lsa.201900346 PubMed 31350258
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