pcDNA3.1/3xmyc_NLRP11-NACHT
(Plasmid
#131199)
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Purposemammalian expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNACHT of NLRP11
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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Mutationcontains only the NACHT domain (amino acids 125-523)
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Entrez GeneNLRP11 (a.k.a. CLR19.6, NALP11, NOD17, PAN10, PYPAF6, PYPAF7)
- Promoter pCMV
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1/3xmyc_NLRP11-NACHT was a gift from Thomas Kufer (Addgene plasmid # 131199 ; http://n2t.net/addgene:131199 ; RRID:Addgene_131199) -
For your References section:
The NLR family pyrin domain containing 11 protein contributes to the regulation of inflammatory signalling. Ellwanger K, Becker E, Kienes I, Sowa A, Postma Y, Gloria YC, Weber ANR, Kufer TA. J Biol Chem. 2018 Jan 4. pii: RA117.000152. doi: 10.1074/jbc.RA117.000152. 10.1074/jbc.RA117.000152 PubMed 29301940
