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Addgene

PYDonly-NLRP11.MYC
(Plasmid #131198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.4
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PYD domain of NLRP11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    384
  • Mutation
    contains only the pyrin domain (amino acids 1-128)
  • Entrez Gene
    NLRP11 (a.k.a. CLR19.6, NALP11, NOD17, PAN10, PYPAF6, PYPAF7)
  • Promoter pCMV
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PYDonly-NLRP11.MYC was a gift from Thomas Kufer (Addgene plasmid # 131198 ; http://n2t.net/addgene:131198 ; RRID:Addgene_131198)

  • For your References section:

    The NLR family pyrin domain containing 11 protein contributes to the regulation of inflammatory signalling. Ellwanger K, Becker E, Kienes I, Sowa A, Postma Y, Gloria YC, Weber ANR, Kufer TA. J Biol Chem. 2018 Jan 4. pii: RA117.000152. doi: 10.1074/jbc.RA117.000152. 10.1074/jbc.RA117.000152 PubMed 29301940
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