pFA NuiHA-loxP-nat-CUP1p
(Plasmid
#131170)
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Purpose(Empty Backbone) PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131170 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA
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Backbone manufacturerWach et. al., 1994
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Vector typeYeast Expression
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Tag
/ Fusion Protein
- Nui-HA (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Nui-HA (N terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA NuiHA-loxP-nat-CUP1p was a gift from Nils Johnsson (Addgene plasmid # 131170 ; http://n2t.net/addgene:131170 ; RRID:Addgene_131170) -
For your References section:
Detecting protein-protein interactions with the Split-Ubiquitin sensor. Dunkler A, Muller J, Johnsson N. Methods Mol Biol. 2012;786:115-30. doi: 10.1007/978-1-61779-292-2_7. 10.1007/978-1-61779-292-2_7 PubMed 21938623
