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Addgene

pExo84-CRU 303
(Plasmid #131165)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131165 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS303
  • Backbone manufacturer
    Sikorski and Hieter, 1991
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ScExo84
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • CRU (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pExo84-CRU 303 was a gift from Nils Johnsson (Addgene plasmid # 131165 ; http://n2t.net/addgene:131165 ; RRID:Addgene_131165)

  • For your References section:

    Detecting protein-protein interactions with the Split-Ubiquitin sensor. Dunkler A, Muller J, Johnsson N. Methods Mol Biol. 2012;786:115-30. doi: 10.1007/978-1-61779-292-2_7. 10.1007/978-1-61779-292-2_7 PubMed 21938623
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