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Addgene

pET-Cdc12-Cdc11
(Plasmid #131162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-Duet
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression ; bicistronic

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    6his-Cdc12 + Cdc11
  • Species
    S. cerevisiae (budding yeast)
  • Promoter T7
  • Tags / Fusion Proteins
    • Cdc12: 6his (N terminal on insert)
    • Cdc11: S-Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Cdc12-Cdc11 was a gift from Nils Johnsson (Addgene plasmid # 131162 ; http://n2t.net/addgene:131162 ; RRID:Addgene_131162)

  • For your References section:

    An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation. Renz C, Johnsson N, Gronemeyer T. BMC Biotechnol. 2013 Jul 26;13:60. doi: 10.1186/1472-6750-13-60. 10.1186/1472-6750-13-60 PubMed 23889817
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