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Addgene

pAc-Cdc3-Cdc10-SNAP
(Plasmid #131161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131161 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC-Duet1
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression ; bicistronic

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cdc3 + Cdc10-SNAP
  • Species
    S. cerevisiae (budding yeast)
  • Promoter T7
  • Tag / Fusion Protein
    • Cdc10: SNAP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

combine with pET-Cdc12-Cdc11 for expression of SNAP-tagged septin rods

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc-Cdc3-Cdc10-SNAP was a gift from Nils Johnsson (Addgene plasmid # 131161 ; http://n2t.net/addgene:131161 ; RRID:Addgene_131161)

  • For your References section:

    An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation. Renz C, Johnsson N, Gronemeyer T. BMC Biotechnol. 2013 Jul 26;13:60. doi: 10.1186/1472-6750-13-60. 10.1186/1472-6750-13-60 PubMed 23889817
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