MigR1-Flag-TRIB3
(Plasmid #131159)

• Purpose: Retroviral expression of flag-tagged mouse TRIB3
• Depositing Labs: Karen Keeshan, Warren Pear
• Publication: Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 10.1182/blood-2009-07-229450. Epub 2010 Apr 21.

Backbone

• Vector backbone: MIGR1
• Backbone manufacturer: Warren Pear (Addgene plasmid # 27490)
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trib3
• Species: M. musculus (mouse)
• Entrez Gene: Trib3 (a.k.a. Ifld2, Nipk, SINK, SKIP3, TRB-3, Trb3)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Trib3 contains a N201T mismatch located within the pseudokinase domain but out-with core motif.

Both Asparagine and Threonine are polar and form hydrogen bonds as both donors and acceptors; substitution should have minimal effect on protein structure/function.