Skip to main content
Addgene

pcDNA-Flag-Trib1
(Plasmid #131156)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trib1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Trib1 (a.k.a. A530090O15Rik, TRB-1, Trb, Trb1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Flag-Trib1 was a gift from Karen Keeshan & Warren Pear (Addgene plasmid # 131156 ; http://n2t.net/addgene:131156 ; RRID:Addgene_131156)

  • For your References section:

    Differential ability of Tribbles family members to promote degradation of C/EBPalpha and induce acute myelogenous leukemia. Dedhia PH, Keeshan K, Uljon S, Xu L, Vega ME, Shestova O, Zaks-Zilberman M, Romany C, Blacklow SC, Pear WS. Blood. 2010 Aug 26;116(8):1321-8. doi: 10.1182/blood-2009-07-229450. Epub 2010 Apr 21. 10.1182/blood-2009-07-229450 PubMed 20410507
Related items:
Commonly requested with: