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Addgene

pcDNA3-Flag-Trib2
(Plasmid #131155)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trib2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Trib2 (a.k.a. AW319517, TRB, TRB-2, TRB2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Flag-Trib2 was a gift from Karen Keeshan & Warren Pear (Addgene plasmid # 131155 ; http://n2t.net/addgene:131155 ; RRID:Addgene_131155)

  • For your References section:

    Tribbles homolog 2 inactivates C/EBPalpha and causes acute myelogenous leukemia. Keeshan K, He Y, Wouters BJ, Shestova O, Xu L, Sai H, Rodriguez CG, Maillard I, Tobias JW, Valk P, Carroll M, Aster JC, Delwel R, Pear WS. Cancer Cell. 2006 Nov;10(5):401-11. doi: 10.1016/j.ccr.2006.09.012. 10.1016/j.ccr.2006.09.012 PubMed 17097562
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