MigR1-Flag-TRIB2
(Plasmid
#131154)
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PurposeRetroviral expression of flag-tagged mouse TRIB2
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 131154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMIGR1
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Backbone manufacturerWarren Pear (Addgene plasmid # 27490)
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Vector typeMammalian Expression, Retroviral
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrib2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1032
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Entrez GeneTrib2 (a.k.a. AW319517, TRB, TRB-2, TRB2)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Murine Trib2 (NM_144551.5); XhoI-ATG-Flag-Trib2-EcoRI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MigR1-Flag-TRIB2 was a gift from Karen Keeshan & Warren Pear (Addgene plasmid # 131154 ; http://n2t.net/addgene:131154 ; RRID:Addgene_131154) -
For your References section:
Tribbles homolog 2 inactivates C/EBPalpha and causes acute myelogenous leukemia. Keeshan K, He Y, Wouters BJ, Shestova O, Xu L, Sai H, Rodriguez CG, Maillard I, Tobias JW, Valk P, Carroll M, Aster JC, Delwel R, Pear WS. Cancer Cell. 2006 Nov;10(5):401-11. doi: 10.1016/j.ccr.2006.09.012. 10.1016/j.ccr.2006.09.012 PubMed 17097562