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MigR1-Myc-Nterm TRIB2
(Plasmid #131153)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131153 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MIGR1
  • Backbone manufacturer
    Warren Pear (Addgene plasmid # 27490)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trib2
  • Species
    M. musculus (mouse)
  • Mutation
    contains amino acids 1-145
  • Entrez Gene
    Trib2 (a.k.a. AW319517, TRB, TRB-2, TRB2)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR from pHMA FL Trib2, N term: 1-145 amino acids = 16 kDa; start-Myc-XhoI-N term-stop-EcoRI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MigR1-Myc-Nterm TRIB2 was a gift from Karen Keeshan & Warren Pear (Addgene plasmid # 131153 ; http://n2t.net/addgene:131153 ; RRID:Addgene_131153)

  • For your References section:

    Tribbles homolog 2 inactivates C/EBPalpha and causes acute myelogenous leukemia. Keeshan K, He Y, Wouters BJ, Shestova O, Xu L, Sai H, Rodriguez CG, Maillard I, Tobias JW, Valk P, Carroll M, Aster JC, Delwel R, Pear WS. Cancer Cell. 2006 Nov;10(5):401-11. doi: 10.1016/j.ccr.2006.09.012. 10.1016/j.ccr.2006.09.012 PubMed 17097562
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