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Addgene

pGL3 T2P 2.6
(Plasmid #131151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    -2.6kb - 0 of mTrib2 upstream of gene transcriptional start site
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    800
  • GenBank ID
    NM_144551.5
  • Entrez Gene
    Trib2 (a.k.a. AW319517, TRB, TRB-2, TRB2)
  • Tag / Fusion Protein
    • luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert contains a A1815T mismatch. This is within a putative CTTTGCAAT CEBP binding site which has not been experimentally validated, and without experimentally validated E2F binding sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3 T2P 2.6 was a gift from Karen Keeshan (Addgene plasmid # 131151 ; http://n2t.net/addgene:131151 ; RRID:Addgene_131151)

  • For your References section:

    Regulation of Trib2 by an E2F1-C/EBPalpha feedback loop in AML cell proliferation. Rishi L, Hannon M, Salome M, Hasemann M, Frank AK, Campos J, Timoney J, O'Connor C, Cahill MR, Porse B, Keeshan K. Blood. 2014 Apr 10;123(15):2389-400. doi: 10.1182/blood-2013-07-511683. Epub 2014 Feb 10. 10.1182/blood-2013-07-511683 PubMed 24516045
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