pGL3 T2P 800
(Plasmid #131147)

• Purpose: To investigate activity of a 800 bp region upstream of mouse Trib2.
• Depositing Lab: Karen Keeshan
• Publication: Rishi et al Blood. 2014 Apr 10;123(15):2389-400. doi: 10.1182/blood-2013-07-511683. Epub 2014 Feb 10.

Backbone

• Vector backbone: pGL3-Basic
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: -800 - 0 of mTrib2 upstream of gene transcriptional start site
• Species: M. musculus (mouse)
• Insert Size (bp): 800
• GenBank ID: NM_144551.5
• Entrez Gene: Trib2 (a.k.a. AW319517, TRB, TRB-2, TRB2)
• Tag / Fusion Protein:
  • luciferase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 800 bp promoter region was cloned by digesting the T2P 2.6 plasmid with KpnI and re-ligating the plasmid with 800bp of the T2P 2.6 still attached to it.

The insert contains a A200T mismatch. This is within a putative CTTTGCAAT CEBP binding site which has not been experimentally validated, and without experimentally validated E2F binding sites.