pGEX-6P-2_dCrk
(Plasmid #131146)

• Purpose: Expresses GST::dCrk (based on crk isoform RA).
• Depositing Lab: Mark Peifer
• Publication: Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302. doi: 10.1091/mbc.E19-05-0302.

Backbone

• Vector backbone: pGEX-6P-2
• Backbone manufacturer: GE Healthcare Life Sciences
• Backbone size w/o insert (bp): 4985
• Total vector size (bp): 5797
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Crk
• Species: D. melanogaster (fly)
• Insert Size (bp): 816
• Entrez Gene: Crk (a.k.a. Dmel_CG1587, CG1587, CRK, D-CRK, D-Crk, DCrk, Dcrk, Dmel\CG1587, crk, dCRK, dCrk)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TTG AAG CTA TCC CAC AAA TTG A
• 3′ sequencing primer: GAA AAA TAA ACA AAT AGG GGT TCC

Resource Information

• Supplemental Documents:
  • pGEX-6P-2_dCrk.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry