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Addgene

phage - ubc - nls - 2xmcp - egfp - cMycBirA*
(Plasmid #131133)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131133 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-UBC
  • Backbone size w/o insert (bp) 6248
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-HA-2xMCP-EGFP-cMycBirA*
  • Alt name
    NLS-HA-2xMCP-EGFP-cMycBioID
  • Species
    Synthetic
  • Insert Size (bp)
    2493
  • Mutation
    BirA* = BIrA >R118G
  • Promoter human ubiquitin C (UBC)
  • Tag / Fusion Protein
    • NLS, HA, cMyc (N-terminal of BirA*) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer 5'-ATTACAGGGACAGCAGAGATCC-3'
  • 3′ sequencing primer WPRE-R (5'CATAGCGTAAAAGGAGCAACA-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Variant of phage - ubc - nls - 2xmcp - egfp - BirA* (Addgene plasmid 131132) with a Myc tag before BirA*

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phage - ubc - nls - 2xmcp - egfp - cMycBirA* was a gift from Ralf Jansen (Addgene plasmid # 131133 ; http://n2t.net/addgene:131133 ; RRID:Addgene_131133)
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