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pET28a Halotag-CNA35
(Plasmid #131128)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131128 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Total vector size (bp) 7181
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 (DE3) for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Halotag-CNA35
  • Alt name
    Halotag-Collagen-binding adhesion protein 35
  • Species
    staphylococcus aureus
  • Insert Size (bp)
    1929
  • Tag / Fusion Protein
    • Halotag (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    https://www.addgene.org/61603/

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modified from https://www.addgene.org/61603/ to express Halotag-CNA35.

Please visit https://www.biorxiv.org/content/10.1101/2022.02.08.479516v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a Halotag-CNA35 was a gift from Samantha Stehbens (Addgene plasmid # 131128 ; http://n2t.net/addgene:131128 ; RRID:Addgene_131128)

  • For your References section:

    A Microtubule Mechanostat Enables Cells to Navigate Confined Environments. Ju RJ, Falconer AD, Tang CKX, Dean KM, Fiolka RP, Sester DP, Nobis M, Timpson P, Lomakin AJ, White MD, Oelz DB, Haass NK, Stehbens SJ. bioRxiv 2022 10.1101/2022.02.08.479516
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