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Addgene

pANTO3
(Plasmid #131107)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131107 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMV261 plasmid
  • Backbone size w/o insert (bp) 3920
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mycobacteriophage L5 integrase
  • Species
    temperate phage of the mycobacteria
  • Insert Size (bp)
    2000

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid has the gene for mycobacteriophage L5 integrase belonging to pMV306 plasmid (Stover CK et al. (1991). Nature 351: 456–460).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence for the vector is that of pMV261 after digestion using NheI- KpnI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pANTO3 was a gift from Riccardo Manganelli (Addgene plasmid # 131107 ; http://n2t.net/addgene:131107 ; RRID:Addgene_131107)

  • For your References section:

    Improving the stability of the TetR/Pip-OFF mycobacterial repressible promoter system. Boldrin F, Anoosheh S, Serafini A, Cioetto Mazzabo L, Palu G, Provvedi R, Manganelli R. Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1038/s41598-019-42319-2. 10.1038/s41598-019-42319-2 PubMed 30962489
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