pANTO5
(Plasmid #131106)

• Purpose: To generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integrase
• Depositing Lab: Riccardo Manganelli
• Publication: Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1038/s41598-019-42319-2.

Backbone

• Vector backbone: pSM128 derived plasmid (the region carrying the attB site and the integrase gene was previously removed using NheI and HindIII)
• Backbone size w/o insert (bp): 7500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: tetR gene by pFRA61 (Boldrin et al 2010)
• Species: E.coli

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid expresses PfurA102tetO pip and tetR genes (Tn10-derived tetR derived by pMC1s plasmid (Ehrt S.et al. (2005). Nucleic Acids Res., 33, e21). The plasmid has also the attP sequence of pMC1s plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid also carries attP sequence belonging to pMC1s plasmid and PfurA 102 tetO- pip belonging to pFRA61 (Boldrin et al 2010).