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Addgene

pAGN33
(Plasmid #131105)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131105 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTTP1.B
  • Backbone manufacturer
    Graham Hatfull (Addgene plasmid # 91723)
  • Backbone size w/o insert (bp) 5852
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    P-furA102tetO-pip
  • Species
    Streptomyces pristinaespiralis
  • Mutation
    the furA102 promoter was mutated with the insertion of 2 tetO sites

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid has PfurA102tetO-pip sequence; PfurA102 pip gene belongs to pMY718 plasmid ( Forti F. et al. (2009). J Biotechnol. Mar 25;140(3-4):270-7); the promoter was modified through the insertion of two TetR operator site ( Ehrt S.et al. (2005). Nucleic Acids Res., 33, e21).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence is that of pTTP1B with P-furA 102 tetO- pip cloned into the EcoRI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGN33 was a gift from Riccardo Manganelli (Addgene plasmid # 131105 ; http://n2t.net/addgene:131105 ; RRID:Addgene_131105)
  • For your References section:

    Improving the stability of the TetR/Pip-OFF mycobacterial repressible promoter system. Boldrin F, Anoosheh S, Serafini A, Cioetto Mazzabo L, Palu G, Provvedi R, Manganelli R. Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1038/s41598-019-42319-2. 10.1038/s41598-019-42319-2 PubMed 30962489