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Addgene

pYJ34-PB-CRISPR-BANCR-E1-gRNA
(Plasmid #131077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-CRISPR
  • Backbone size w/o insert (bp) 11659
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BANCR Exon1 gRNA
  • gRNA/shRNA sequence
    GAAATAGACTGCAGCACCAA
  • Species
    H. sapiens (human)
  • Entrez Gene
    BANCR (a.k.a. LINC00586)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYJ34-PB-CRISPR-BANCR-E1-gRNA was a gift from Xiaojun Lian (Addgene plasmid # 131077 ; http://n2t.net/addgene:131077 ; RRID:Addgene_131077)

  • For your References section:

    Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems. Jiang Y, Hoenisch RC, Chang Y, Bao X, Cameron CE, Lian XL. Bioact Mater. 2022 Feb 7;14:313-320. doi: 10.1016/j.bioactmat.2022.01.046. eCollection 2022 Aug. 10.1016/j.bioactmat.2022.01.046 PubMed 35386818
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