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pYJ21-lenti-EF1a-ASAP2f-puro
(Plasmid #131065)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131065 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXL001
  • Backbone size w/o insert (bp) 7569
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASAP2f
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1281

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a Y141N mutation in ASAP2f. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYJ21-lenti-EF1a-ASAP2f-puro was a gift from Xiaojun Lian (Addgene plasmid # 131065 ; http://n2t.net/addgene:131065 ; RRID:Addgene_131065)

  • For your References section:

    A dual cardiomyocyte reporter model derived from human pluripotent stem cells. Jiang Y, Bao X, Lian XL. Stem Cell Res Ther. 2021 May 29;12(1):305. doi: 10.1186/s13287-021-02341-6. 10.1186/s13287-021-02341-6 PubMed 34051863
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