pYJ21-lenti-EF1a-ASAP2f-puro
(Plasmid
#131065)
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PurposeForced expression of ASAP2f
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXL001
- Backbone size w/o insert (bp) 7569
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameASAP2f
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SpeciesH. sapiens (human)
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Insert Size (bp)1281
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a Y141N mutation in ASAP2f. This mutation is not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYJ21-lenti-EF1a-ASAP2f-puro was a gift from Xiaojun Lian (Addgene plasmid # 131065 ; http://n2t.net/addgene:131065 ; RRID:Addgene_131065) -
For your References section:
A dual cardiomyocyte reporter model derived from human pluripotent stem cells. Jiang Y, Bao X, Lian XL. Stem Cell Res Ther. 2021 May 29;12(1):305. doi: 10.1186/s13287-021-02341-6. 10.1186/s13287-021-02341-6 PubMed 34051863
