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HP21-TPI-WT-no_introns
(Plasmid #131044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TPI-Wildtype-no introns
  • Species
    H. sapiens (human)
  • Entrez Gene
    TPI1 (a.k.a. HEL-S-49, TIM, TPI, TPID)
  • Promoter Pcmv

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid has a 21 nt hairpin in 5'UTR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HP21-TPI-WT-no_introns was a gift from Marvin Tanenbaum (Addgene plasmid # 131044 ; http://n2t.net/addgene:131044 ; RRID:Addgene_131044)

  • For your References section:

    Single-Molecule Imaging Uncovers Rules Governing Nonsense-Mediated mRNA Decay. Hoek TA, Khuperkar D, Lindeboom RGH, Sonneveld S, Verhagen BMP, Boersma S, Vermeulen M, Tanenbaum ME. Mol Cell. 2019 May 23. pii: S1097-2765(19)30361-2. doi: 10.1016/j.molcel.2019.05.008. 10.1016/j.molcel.2019.05.008 PubMed 31155380
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