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K8.1Pr-pGL4.16-OriLyt
(Plasmid #131038)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131038 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.16
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6075
  • Total vector size (bp) 9648
  • Modifications to backbone
    100 bp of the KSHV K8.1 Promoter was cloned between the KpnI and HindIII restriction sites
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KSHV Lytic Origin of replication
  • Species
    Kaposi's Sarcoma Herpesvirus
  • Insert Size (bp)
    3625

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstXI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CTTGGTCTGACAGCGGCCG
  • 3′ sequencing primer AGCACTGGTAACCACTGCAGTGGaccgacctatgcgcgcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K8.1Pr-pGL4.16-OriLyt was a gift from Britt Glaunsinger (Addgene plasmid # 131038 ; http://n2t.net/addgene:131038 ; RRID:Addgene_131038)

  • For your References section:

    An integrative approach identifies direct targets of the late viral transcription complex and an expanded promoter recognition motif in Kaposi's sarcoma-associated herpesvirus. Nandakumar D, Glaunsinger B. PLoS Pathog. 2019 May 16;15(5):e1007774. doi: 10.1371/journal.ppat.1007774. eCollection 2019 May. 10.1371/journal.ppat.1007774 PubMed 31095645
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