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pcDNA3.1(+)-OTI-TCRA_p2_TCRB
(Plasmid #131033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7235
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    TCRA
  • Alt name
    OT-I TCRA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tcra (a.k.a. Tcralpha)
  • Promoter CMV/T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TCRB
  • Alt name
    OT-I TCRB
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tcrb (a.k.a. TCRbeta, Tib)
  • Promoter CMV/T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GenScript. Also Addgene Plasmid 52111 from Dario Vignali's lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/466243v5 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)-OTI-TCRA_p2_TCRB was a gift from Jeff Hammerbacher (Addgene plasmid # 131033 ; http://n2t.net/addgene:131033 ; RRID:Addgene_131033)

  • For your References section:

    Viable and efficient electroporation-based genetic manipulation of unstimulated human T cells. Aksoy P, Aksoy Bulent Arman, Czech E, Hammerbacher J. bioRxiv 10.1101/466243
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