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Addgene

pcF-1
(Plasmid #131023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD18
  • Total vector size (bp) 3443
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    single spacer array for FnCas12a
  • Insert Size (bp)
    102
  • Promoter PJ23119

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AGCGCTCATGAGCCCGA
  • 3′ sequencing primer TGCCGCCAGGCAAATTCTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcF-1 was a gift from Chase Beisel (Addgene plasmid # 131023 ; http://n2t.net/addgene:131023 ; RRID:Addgene_131023)

  • For your References section:

    Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis. Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil TD, Leenay RT, Keung AJ, Beisel CL. Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3. 10.1038/s41467-019-10747-3 PubMed 31270316
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