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Addgene

pHWBF01
(Plasmid #131022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 131022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 2686
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H2B.5
  • Species
    Zea mays (maize)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Synthesized by Genscript. This clone has same protein sequence as reported in Howe et al, 2012. Pubmed ID: 22662764

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHWBF01 was a gift from Hank Bass (Addgene plasmid # 131022 ; http://n2t.net/addgene:131022 ; RRID:Addgene_131022)

  • For your References section:

    Maize histone H2B-mCherry: a new fluorescent chromatin marker for somatic and meiotic chromosome research. Howe ES, Clemente TE, Bass HW. DNA Cell Biol. 2012 Jun;31(6):925-38. doi: 10.1089/dna.2011.1514. Epub 2012 Jun 4. 10.1089/dna.2011.1514 PubMed 22662764
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