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Addgene

NCH2exp
(Plasmid #131019)

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Item Catalog # Description Quantity Price (USD)
Plasmid 131019 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pH7WG2
  • Backbone size w/o insert (bp) 11546
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NCH2
  • Species
    Zea mays (maize)
  • Promoter P35S
  • Tag / Fusion Protein
    • eGFP-FLAG-HA (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NCH2exp was a gift from Hank Bass (Addgene plasmid # 131019 ; http://n2t.net/addgene:131019 ; RRID:Addgene_131019)

  • For your References section:

    Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear Envelope Remodeling and Function in Stomatal Complex Development and Pollen Viability. McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann K, Bass HW. Front Plant Sci. 2021 Feb 17;12:645218. doi: 10.3389/fpls.2021.645218. eCollection 2021. 10.3389/fpls.2021.645218 PubMed 33679862
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