pAsCpf1GG
(Plasmid #131011)

• Purpose: Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
• Depositing Lab: Chase Beisel
• Publication: Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3.

Backbone

• Vector backbone: pBAD18
• Total vector size (bp): 4410
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: direct repeat of AsCas12a
• Insert Size (bp): 35

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: AGCGCTCATGAGCCCGA
• 3′ sequencing primer: TGCCGCCAGGCAAATTCTG

Gene/Insert 2

• Gene/Insert name: GFP expression cassette
• Insert Size (bp): 1012

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: AGCGCTCATGAGCCCGA
• 3′ sequencing primer: TGCCGCCAGGCAAATTCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry