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pET22b-mEB2-eGFP-6xHis
(Plasmid #130982)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EB2-GFP-6xHis
  • Alt name
    Mapre2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mapre2 (a.k.a. C820009F03Rik, D18Abb1e, EB1, EB2, RP1)
  • Promoter T7
  • Tag / Fusion Protein
    • eGFP-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b-mEB2-eGFP-6xHis was a gift from Anne Straube (Addgene plasmid # 130982 ; http://n2t.net/addgene:130982 ; RRID:Addgene_130982)

  • For your References section:

    Spatial positioning of EB family proteins at microtubule tips involves distinct nucleotide-dependent binding properties. Roth D, Fitton BP, Chmel NP, Wasiluk N, Straube A. J Cell Sci. 2018 Oct 31;132(4). pii: jcs.219550. doi: 10.1242/jcs.219550. 10.1242/jcs.219550 PubMed 30262468
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