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Addgene

pKIF1C-2xFLAG
(Plasmid #130979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF1C-2xFLAG
  • Alt name
    KIF1C kinesin family member 1C
  • Species
    H. sapiens (human)
  • Mutation
    4 silent mutations that make this construct resistant to RNAi oligo siKIF1C-1 also used in this paper. Nucleotides 649-663 (TCCCATGCCGTCTTT) have been replaced with TCGCACGCAGTCTTC.
  • Entrez Gene
    KIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKIF1C-2xFLAG was a gift from Anne Straube (Addgene plasmid # 130979 ; http://n2t.net/addgene:130979 ; RRID:Addgene_130979)

  • For your References section:

    Directional persistence of migrating cells requires Kif1C-mediated stabilization of trailing adhesions. Theisen U, Straube E, Straube A. Dev Cell. 2012 Dec 11;23(6):1153-66. doi: 10.1016/j.devcel.2012.11.005. 10.1016/j.devcel.2012.11.005 PubMed 23237952
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