pKIF1C-mCherry
(Plasmid
#130978)
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PurposeExpression of KIF1C-mCherry in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 130978 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKIF1C-mCherry
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Alt nameKIF1C kinesin family member 1C
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SpeciesH. sapiens (human)
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Entrez GeneKIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKIF1C-mCherry was a gift from Anne Straube (Addgene plasmid # 130978 ; http://n2t.net/addgene:130978 ; RRID:Addgene_130978) -
For your References section:
Directional persistence of migrating cells requires Kif1C-mediated stabilization of trailing adhesions. Theisen U, Straube E, Straube A. Dev Cell. 2012 Dec 11;23(6):1153-66. doi: 10.1016/j.devcel.2012.11.005. 10.1016/j.devcel.2012.11.005 PubMed 23237952