pKIF1C-GFP
(Plasmid #130977)

• Purpose: Expression of KIF1C-GFP in mammalian cells.
• Depositing Lab: Anne Straube
• Publication: Efimova et al J Cell Sci. 2014 Dec 15;127(Pt 24):5179-88. doi: 10.1242/jcs.149633. Epub 2014 Oct 24.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIF1C-GFP
• Alt name: KIF1C kinesin family member 1C
• Species: H. sapiens (human)
• Mutation: 5 silent mutations that make this construct RNAi resistant to siKIF1C-2 also in this paper. Nucleotides 433-444 are changed from AGCTATATGGAG to TCTTAGATGGAA.
• Entrez Gene: KIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • addgene_id_130977_pKIF1C-GFP.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry