Skip to main content
Addgene

pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf
(Plasmid #130976)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFastBacM13
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hook3-SNAPf
  • Species
    H. sapiens (human)
  • Entrez Gene
    HOOK3 (a.k.a. HK3)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • 8His-ZZ-LTLT (N terminal on insert)
    • SNAPf (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf was a gift from Anne Straube (Addgene plasmid # 130976 ; http://n2t.net/addgene:130976 ; RRID:Addgene_130976)

  • For your References section:

    PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport. Siddiqui N, Zwetsloot AJ, Bachmann A, Roth D, Hussain H, Brandt J, Kaverina I, Straube A. Nat Commun. 2019 Jun 19;10(1):2693. doi: 10.1038/s41467-019-10644-9. 10.1038/s41467-019-10644-9 PubMed 31217419
Related items:
Commonly requested with: