pFastBac-M13-6His-KIF1C-GFP
(Plasmid #130975)

• Purpose: Expression of 6His-KIF1C-GFP in insect cells for protein purification.
• Depositing Lab: Anne Straube
• Publication: Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi: 10.1038/s41467-019-10644-9.

Backbone

• Vector backbone: pFastBacM13
• Backbone manufacturer: ThermoFisher
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIF1C-GFP
• Alt name: KIF1C kinesin family member 1C
• Species: H. sapiens (human)
• Mutation: 5 silent mutations that make this construct RNAi resistant to siKIF1C-2 also used in this paper. Nucleotides 433-444 are changed from AGCTATATGGAG to TCTTAGATGGAA.
• Entrez Gene: KIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
• Promoter: Polyhedrin
• Tags / Fusion Proteins:
  • 6His (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • addgene_id_130975_pFastM13_cterm_Kif1cGFPMBN.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry