pHCT2
(Plasmid
#130970)
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PurposeHyPr plasmid with natMX marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 130970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHCT2
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Vector typeYeast Expression
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Selectable markersnourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionslow copy (CEN) in yeast
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHCT2 was a gift from Chris Todd Hittinger (Addgene plasmid # 130970 ; http://n2t.net/addgene:130970 ; RRID:Addgene_130970) -
For your References section:
Synthetic hybrids of six yeast species. Peris D, Alexander WG, Fisher KJ, Moriarty RV, Basuino MG, Ubbelohde EJ, Wrobel RL, Hittinger CT. Nat Commun. 2020 Apr 29;11(1):2085. doi: 10.1038/s41467-020-15559-4. 10.1038/s41467-020-15559-4 PubMed 32350251