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HI0058/pET-21a(+)c-tag
(Plasmid #13097)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13097 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21a(+)
  • Backbone manufacturer
    Merck
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    3-deoxy-manno-octulosonate cytidylyltransferase
  • Alt name
    KDSB_HAEIN
  • Species
    Haemophilus influenzae
  • Insert Size (bp)
    765
  • GenBank ID
    NC_000907
  • Entrez Gene
    kdsB
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HI0058/pET-21a(+)c-tag was a gift from SeWon Suh (Addgene plasmid # 13097 ; http://n2t.net/addgene:13097 ; RRID:Addgene_13097)

  • For your References section:

    Crystallization and preliminary X-ray crystallographic studies of 3-deoxy-manno-octulosonate cytidylyltransferase from Haemophilus influenzae. Ku MJ, Yoon HJ, Ahn HJ, Kim HW, Baek SH, Suh SW. Acta Crystallogr D Biol Crystallogr. 2003 Jan . 59(Pt 1):180-2. 10.1107/S0907444902019698 PubMed 12499564
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