pET-His6-FnCas9GFP
(Plasmid #130966)

• Purpose: expression of FnCas9-GFP in bacterial cells
• Depositing Lab: Debojyoti Chakraborty
• Publication: Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 10.1073/pnas.1818461116. Epub 2019 Sep 30.

Backbone

• Vector backbone: pET-His6-GFP-TEV-LIC
• Backbone size w/o insert (bp): 6082
• Total vector size (bp): 11029
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HA-NLS-FnCas9
• Alt name: Francisella tularensis subsp. novicida Cas9 (FtCas9), Francisella novicida Cas9 (FnoCas9)
• Alt name: FnCas9b
• Species: Synthetic; Francisella tularensis subsp. novicida
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-mEGFP-TEV-HA-NLS (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: GFP forward (5'CAGCTCGCCGACCACTA3')

Resource Information

• A portion of this plasmid was derived from a plasmid made by: FnCas9 gene was subcloned from PX408, a gift from Feng Zhang

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry