Skip to main content
Addgene

pLJM1-zeo-ORF30-2xStrep
(Plasmid #130958)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLJM1
  • Backbone size w/o insert (bp) 7160
  • Total vector size (bp) 7391
  • Vector type
    Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ORF30
  • Species
    Kaposi's Sarcoma Associated Herpesvirus (HHV-8)
  • Insert Size (bp)
    231
  • Entrez Gene
    ORF30 (a.k.a. HHV8GK18_gp34)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xStrep (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer ggatctctgctgtccctgtaataaacc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1-zeo-ORF30-2xStrep was a gift from Britt Glaunsinger (Addgene plasmid # 130958 ; http://n2t.net/addgene:130958 ; RRID:Addgene_130958)

  • For your References section:

    Conserved CxnC motifs in Kaposi's sarcoma-associated herpesvirus ORF66 are required for viral late gene expression and are essential for its interaction with ORF34. Didychuk AL, Castaneda AF, Kushnir LO, Huang CJ, Glaunsinger BA. J Virol. 2019 Oct 2. pii: JVI.01299-19. doi: 10.1128/JVI.01299-19. 10.1128/JVI.01299-19 PubMed 31578296
Related items: