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pCRII-Blunt-U6-HES7-STOP-gRNA
(Plasmid #130933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-bluntII
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 3519
  • Total vector size (bp) 3974
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HES7-STOP-gRNA
  • Alt name
    HES7
  • Species
    H. sapiens (human)
  • Entrez Gene
    HES7 (a.k.a. SCDO4, bHLHb37, hHes7)
  • Promoter U6

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGTG
  • 3′ sequencing primer GGAAACAGCTATGACCATG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII-Blunt-U6-HES7-STOP-gRNA was a gift from James Thomson (Addgene plasmid # 130933 ; http://n2t.net/addgene:130933 ; RRID:Addgene_130933)

  • For your References section:

    An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells. Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, Stewart R, Thomson JA. Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 10.1016/j.celrep.2019.07.090. 10.1016/j.celrep.2019.07.090 PubMed 31461642
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