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Addgene

HES7-NLuc-2A-tdTomato
(Plasmid #130932)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDNR
  • Total vector size (bp) 10433
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HES7
  • Alt name
    HES7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1760
  • Entrez Gene
    HES7 (a.k.a. SCDO4, bHLHb37, hHes7)
  • Tag / Fusion Protein
    • NanoLuc and tdTomato

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcttggactcctgttgataga
  • 3′ sequencing primer ctctgacttgagcgtcgattt
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HES7-NLuc-2A-tdTomato was a gift from James Thomson (Addgene plasmid # 130932 ; http://n2t.net/addgene:130932 ; RRID:Addgene_130932)

  • For your References section:

    An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells. Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, Stewart R, Thomson JA. Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 10.1016/j.celrep.2019.07.090. 10.1016/j.celrep.2019.07.090 PubMed 31461642
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