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Addgene

pLenti-CMV-cGAS R71/75E C396/7A-HA
(Plasmid #130920)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti CMV GFP Puro
  • Backbone manufacturer
    Addgene (#17448)
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 9473
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human cGAS R71/75E C396/7A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    aa 71 and 75 mutated to glutamate; aa 396 and 397 mutated to alanine
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-CMV-cGAS R71/75E C396/7A-HA was a gift from Jonathan Kagan (Addgene plasmid # 130920 ; http://n2t.net/addgene:130920 ; RRID:Addgene_130920)

  • For your References section:

    Phosphoinositide Interactions Position cGAS at the Plasma Membrane to Ensure Efficient Distinction between Self- and Viral DNA. Barnett KC, Coronas-Serna JM, Zhou W, Ernandes MJ, Cao A, Kranzusch PJ, Kagan JC. Cell. 2019 Mar 7;176(6):1432-1446.e11. doi: 10.1016/j.cell.2019.01.049. Epub 2019 Feb 28. 10.1016/j.cell.2019.01.049 PubMed 30827685
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