Skip to main content
Addgene

pAAV-hSyn-DIO-jGCamp7b-P2A-GSG-mRuby3
(Plasmid #130900)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 130900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4222
  • Total vector size (bp) 6960
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    jGCaMP7b-P2A-mRuby3
  • Species
    Synthetic
  • Insert Size (bp)
    2738
  • GenBank ID
    ATE88097 QCW12733
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer TGTCGTGCCTGAGAGCGCAGTC
  • 3′ sequencing primer GTAATCCAGAGGTTGATTATCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-DIO-jGCamp7b-P2A-GSG-mRuby3 was a gift from Simon Wiegert (Addgene plasmid # 130900 ; http://n2t.net/addgene:130900 ; RRID:Addgene_130900)
Related items:
Commonly requested with: