Skip to main content
Addgene

hTLR5 flag
(Plasmid #13088)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13088 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFlag-CMV-1
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TLR5
  • Alt name
    toll-like receptor 5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Mutation
    Amino acids 1-19 have been replaced by a mammalian signal sequence (SALLILALVGAAVA) followed by Flag tag
  • GenBank ID
    NM_003268
  • Entrez Gene
    TLR5 (a.k.a. MELIOS, SLE1, SLEB1, TIL3)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV30
  • 3′ sequencing primer CMV24
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hTLR5 flag was a gift from Ruslan Medzhitov (Addgene plasmid # 13088 ; http://n2t.net/addgene:13088 ; RRID:Addgene_13088)
Related items:
Commonly requested with: